Paper

2024

 

Nießing, Bastian; Breitkreuz, Yannick; Elanzew, Andreas; Szymanski De Toledo, Marcelo; Vajs, Peter; Nolden, Marina; Erkens, Frederik; Wanek, Paul; Au Yeung, Si Wah Christina; Haupt, Simone; König, Niels; Peitz, Michael; Schmitt, Robert; Zenke, Martin; Brüstle, Oliver. (2024): Automated CRISPR/Cas9-based genome editing of human pluripotent stem cells using the StemCellFactory. In: Frontiers in Bioengineering and Biotechnology 12, Artikel 1459273. DOI: 10.3389/fbioe.2024.1459273.

 

 

2022

 

Ma, Zhiyao; Toledo, Marcelo Augusto Szymanskide; Wanek, Paul; Elsafi Mabrouk, Mohamed H.; Smet, Francis; Pulak, Rock et al. (2022):
Cell Cluster Sorting in Automated Differentiation of Patient-specific Induced Pluripotent Stem Cells Towards Blood Cells.

In: Front. Bioengineering and Biotechnology 10, Artikel 755983. DOI: 10.3389/fbioe.2022.755983. 

 

 

2021

 

Biermann, Ferdinand; Mathews, Julius; Nießing, Bastian; König, Niels; Schmitt, Robert H. (2021):

Automating Laboratory Processes by Connecting Biotech and Robotic Devices—An Overview of the Current Challenges, Existing Solutions and Ongoing Developments.
In: Processes 9 (6), Artikel 966. DOI: 10.3390/pr9060966.

 

Nießing, Bastian; Kiesel, Raphael; Herbst, Laura; Schmitt, Robert H. (2021):
Techno-Economic Analysis of Automated iPSC Production.
In: Processes 9 (2), Artikel 240. DOI: 10.3390/pr9020240.

 

Piotrowski, Tobias; Rippel, Oliver; Elanzew, Andreas; Nießing, Bastian; Stucken, Sebastian; Jung, Sven et al. (2021):
Deep-learning-based multi-class segmentation for automated, non-invasive routine assessment of human pluripotent stem cell culture status.

In: Comput. Biol. Med. 129, Artikel 104172. DOI: 10.1016/j.compbiomed.2020.104172. 

 

Toledo, Marcelo A. S.; Gatz, Malrun; Sontag, Stephanie; Gleixner, Karoline V.; Eisenwort, Gregor; Feldberg, Kristina et al. (2021):

Nintedanib targets KIT D816V neoplastic cells derived from induced pluripotent stem cells of systemic mastocytosis.

In: Blood 137 (15), S. 2070–2084. DOI: 10.1182/blood.2019004509.

 

 

Satoh, Taiki; Toledo, Marcelo A.S.; Boehnke, Janik, Olschok, Kathrin; Flosdorf, Niclas, Götz, Katrin; Küstermann, Caroline; Sontag, Stephanie; Seré, Kristi; Koschmieder, Steffen; Brümmendorf, Chatain, Nicolas; Tagawa, Yoh-ichi; Zenke, Martin (2021): Human DC3 Antigen Presenting Dendritic Cells From Induced Pluripotent Stem Cells.

In: Frontiers in Cell and Developmental Biology 21(9). DOI:0.3389/fcell.2021.667304.

 

 

 

2020

 

Doulgkeroglou, Meletios-Nikolaos; Di Nubila, Alessia; Niessing, Bastian; König, Niels; Schmitt, Robert H.; Damen, Jackie et al. (2020): Automation, Monitoring, and Standardization of Cell Product Manufacturing.

In: Front. Bioeng. Biotechnol. 8, Artikel 811. DOI: 10.3389/fbioe.2020.00811.

 

Elanzew, Andreas; Nießing, Bastian; Langendoerfer, Daniel; Rippel, Oliver; Piotrowski, Tobias; Schenk, Friedrich et al. (2020):

The StemCellFactory: A Modular System Integration for Automated Generation and Expansion of Human Induced Pluripotent Stem Cells.

In: Front. Bioeng. Biotechnol. 8, Artikel 580352. DOI: 10.3389/fbioe.2020.580352.

 

Lampert, Angelika; Bennett, David L.; McDermott, Lucy A.; Neureiter, Anika; Eberhardt, Esther; Winner, Beate; Zenke, Martin (2020):

Human sensory neurons derived from pluripotent stem cells for disease modelling and personalized medicine.

In: Neurobiol. Pain 8, Artikel 100055. DOI: 10.1016/j.ynpai.2020.100055. 

 

 

2019

 

Meents, Jannis E.; Bressan, Elisangela; Sontag, Stephanie; Foerster, Alec; Hautvast, Petra; Rösseler, Corinna et al. (2019):

The role of Nav1.7 in human nociceptors: insights from human induced pluripotent stem cell-derived sensory neurons of erythromelalgia patients.

In: Pain 160 (6), S. 1327–1341. DOI: 10.1097/j.pain.0000000000001511. 

 

 

2018

 

Jung, Sven; Ochs, Jelena; Kulik, Michael; König, Niels; Schmitt, Robert H. (2018):

Highly modular and generic control software for adaptive cell processing on automated production platforms.

In: Procedia CIRP 72, S. 1245–1250. DOI: 10.1016/j.procir.2018.03.189. 

 

 

2017

 

Badenes, Sara M.; Fernandes, Tiago G.; Miranda, Cláudia C.; Pusch-Klein, Annette; Haupt, Simone; Rodrigues, Carlos A. V. et al. (2017):

Long-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system.

In: J. Chem. Technol. Biotechnol. 92 (3), S. 492–503. DOI: 10.1002/jctb.5074.

 

Jungverdorben, Johannes; Till, Andreas; Brüstle, Oliver (2017):

Induced pluripotent stem cell-based modeling of neurodegenerative diseases: a focus on autophagy.

In: J. Mol. Med. 95 (7), S. 705–718. DOI: 10.1007/s00109-017-1533-5.

 

Kulik, Michael; Ochs, Jelena; König, Niels; McBeth, Christine; Sauer-Budge, Alexis; Sharon, Andre; Schmitt, Robert (2017):

Parallelization in Automated Stem Cell Culture.

In: Procedia CIRP 65, S. 242–247. DOI: 10.1016/j.procir.2017.04.053.

 

Roese-Koerner, Beate; Stappert, Laura; Brüstle, Oliver (2017):

Notch/Hes signaling and miR-9 engage in complex feedback interactions controlling neural progenitor cell proliferation and differentiation. In: Neurogenesis 4 (1), Artikel e1313647. DOI: 10.1080/23262133.2017.1313647.

 

Sontag, Stephanie; Förster, Malrun; Qin, Jie; Wanek, Paul; Mitzka, Saskia; Schüler, Herdit M. et al. (2017):

Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells.

In: Stem Cells 35 (4), S. 898–908. DOI: 10.1002/stem.2565.

 

Sontag, Stephanie; Förster, Malrun; Seré, Kristin; Zenke, Martin (2017):

Differentiation of Human Induced Pluripotent Stem Cells (iPS Cells) and Embryonic Stem Cells (ES Cells) into Dendritic Cell (DC) Subsets.

In: Bio-protoc. 7 (15), Artikel e2419. DOI: 10.21769/BioProtoc.2419. 

 

 

2016

 

Falk, Anna; Heine, Vivi M.; Harwood, Adrian J.; Sullivan, P. F.; Peitz, Michael; Brüstle, Oliver et al. (2016):

Modeling psychiatric disorders: from genomic findings to cellular phenotypes.

In: Mol. Psychiatry 21 (9), S. 1167–1179. DOI: 10.1038/mp.2016.89.

 

Heesen, Ludwig; Peitz, Michael; Torres-Benito, Laura; Hölker, Irmgard; Hupperich, Kristina; Dobrindt, Kristina et al. (2016):

Plastin 3 is upregulated in iPSC-derived motoneurons from asymptomatic SMN1-deleted individuals.

In: Cell. Mol. Life Sci. 73 (10), S. 2089–2104. DOI: 10.1007/s00018-015-2084-y.

 

Kulik, Michael; Elanzew, Andreas; Haupt, Simone; Wanek, Paul; Ochs, Jelena; Konig, Niels et al. (2016):

Novel platform for fully automated generation and expansion of highly standardized iPS cells.

In: J. Biotechnol. 231, Supplement, S33-S34. DOI: 10.1016/j.jbiotec.2016.05.136.

 

Roese-Koerner, Beate; Stappert, Laura; Berger, Thomas; Braun, Nils Christian; Veltel, Monika; Jungverdorben, Johannes et al. (2016): Reciprocal Regulation between Bifunctional miR-9/9(∗) and its Transcriptional Modulator Notch in Human Neural Stem Cell Self-Renewal and Differentiation.

In: Stem Cell Rep. 7 (2), S. 207–219. DOI: 10.1016/j.stemcr.2016.06.008.

 

Schenk, Friedrich Walter; Brill, Nicolai; Marx, Ulrich; Hardt, Daniel; König, Niels; Schmitt, Robert (2016):

High-speed microscopy of continuously moving cell culture vessels.

In: Sci. Rep. 6, Artikel 34038. DOI: 10.1038/srep34038. 

 

 

2015

 

Elanzew, Andreas; Sommer, Annika; Pusch-Klein, Annette; Brüstle, Oliver; Haupt, Simone (2015):

A reproducible and versatile system for the dynamic expansion of human pluripotent stem cells in suspension.

In: Biotechnol. J. 10 (10), S. 1589–1599. DOI: 10.1002/biot.201400757.

 

Friedrichs, Stephanie; Malan, Daniela; Voss, Yvonne; Sasse, Philipp (2015):

Scalable Electrophysiological Investigation of iPS Cell-Derived Cardiomyocytes Obtained by a Lentiviral Purification Strategy.

In: J. Clin. Med. 4 (1), S. 102–123. DOI: 10.3390/jcm4010102.

 

Kadari, Asifiqbal; Mekala, SubbaRao; Wagner, Nicole; Malan, Daniela; Köth, Jessica; Doll, Katharina et al. (2015):

Robust Generation of Cardiomyocytes from Human iPS Cells Requires Precise Modulation of BMP and WNT Signaling.

In: Stem Cell Rev. Rep. 11 (4), S. 560–569. DOI: 10.1007/s12015-014-9564-6.

 

Lenz, Michael; Goetzke, Roman; Schenk, Arne; Schubert, Claudia; Veeck, Jürgen; Hemeda, Hatim et al. (2015):

Epigenetic Biomarker to Support Classification into Pluripotent and Non-Pluripotent Cells.

In: Sci. Rep. 5, Artikel 8973. DOI: 10.1038/srep08973.

 

Schenk, Friedrich W.; Kulik, Michael; Schmitt, Robert (2015):

Metrology-based quality and process control in automated stem cell production.

In: TM. Tech. Mess. 82 (6), S. 309–316. DOI: 10.1515/teme-2015-0036.

 

Zhang, Miao; Schulte, Jan Sebastian; Heinick, Alexander; Piccini, Ilaria; Rao, Jyoti; Quaranta, Roberto et al. (2015):

Universal Cardiac Induction of Human Pluripotent Stem Cells in Two and Three-Dimensional Formats: Implications for In Vitro Maturation. In: Stem Cells 33 (5), S. 1456–1469. DOI: 10.1002/stem.1964. 

 

 

2014

 

Frobel, Joana; Hemeda, Hatim; Lenz, Michael; Abagnale, Giulio; Joussen, Sylvia; Denecke, Bernd et al. (2014):

Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells.

In: Stem Cell Rep. 3 (3), S. 414–422. DOI: 10.1016/j.stemcr.2014.07.003.

 

Qin, Jie; Sontag, Stephanie; Lin, Qiong; Mitzka, Saskia; Leisten, Isabelle; Schneider, Rebekka K. et al. (2014):

Cell fusion enhances mesendodermal differentiation of human induced pluripotent stem cells.

In: Stem Cells Dev. 23 (23), S. 2875–2882. DOI: 10.1089/scd.2014.0120.

 

 

2013

 

 

Koch, Carmen M.; Reck, Kristina; Shao, Kaifeng; Lin, Qiong; Joussen, Sylvia; Ziegler, Patrick et al. (2013):

Pluripotent stem cells escape from senescence-associated DNA methylation changes.

In: Genome Res. 23 (2), S. 248–259. DOI: 10.1101/gr.141945.112.

 

Lenz, Michael; Schuldt, Bernhard M.; Müller, Franz-Josef; Schuppert, Andreas (2013):

PhysioSpace: Relating Gene Expression Experiments from Heterogeneous Sources Using Shared Physiological Processes.

In: PloS ONE 8 (10), Artikel e77627. DOI: 10.1371/journal.pone.0077627.

 

Marx, Ulrich; Schenk, Friedrich; Behrens, Jan; Meyr, Ulrike; Wanek, Paul; Zang, Werner et al. (2013):

Automatic Production of Induced Pluripotent Stem Cells.

In: Procedia CIRP 5, S. 2–6. DOI: 10.1016/j.procir.2013.01.001.

 

McLaren, Donna; Gorba, Thorsten; Marguerie de Rotrou, Anita; Pillai, Gopalan; Chappell, Clare; Stacey, Alison et al. (2013):

Automated Large-Scale Culture and Medium-Throughput Chemical Screen for Modulators of Proliferation and Viability of Human Induced Pluripotent Stem Cell–Derived Neuroepithelial-like Stem Cells.

In: J. Biomol. Screen. 18 (3), S. 258–268. DOI: 10.1177/1087057112461446.

 

Peitz, Michael; Jungverdorben, Johannes; Brüstle, Oliver (2013):

Disease-specific iPS cell models in neuroscience.

In: Curr. Mol. Med. 13 (5), S. 832–841. DOI: 10.2174/1566524011313050014.

 

Schellenberg, Anne; Hemeda, Hatim; Wagner, Wolfgang (2013):

Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency.

In: Kursad Turksen (Hg.): Stem Cells and Aging. Methods and Protocols. Totowa, NJ: Humana (Methods in Molecular Biology, 976), S. 143–154.

 

Shao, Kaifeng; Koch, Carmen; Gupta, Manoj K.; Lin, Qiong; Lenz, Michael; Laufs, Stephanie et al. (2013):

Induced Pluripotent Mesenchymal Stromal Cell Clones Retain Donor-derived Differences in DNA Methylation Profiles.

In: Mol. Ther. 21 (1), S. 240–250. DOI: 10.1038/mt.2012.207.

 

Turksen, Kursad (Hg.) (2013):

Stem Cells and Aging. Methods and Protocols. Totowa, NJ: Humana (Methods in Molecular Biology, 976).

 

Willmann, Charlotte A.; Hemeda, Hatim; Pieper, Lisa A.; Lenz, Michael; Qin, Jie; Joussen, Sylvia et al. (2013):

To Clone or Not to Clone? Induced Pluripotent Stem Cells Can Be Generated in Bulk Culture.

In: PloS ONE 8 (5), Artikel e65324. DOI: 10.1371/journal.pone.0065324.

 

2012

 

Haupt, Simone; Grützner, Jan; Thier, Marc-Christian; Kallweit, Tobias; Rath, Barbara Helen; Laufenberg, Iris et al. (2012):

Automated selection and harvesting of pluripotent stem cell colonies.

In: Biotechnol. Appl. Biochem. 59 (2), S. 77–87. DOI: 10.1002/bab.1014.

 

Haupt, Simone; Wanek, Paul; Marx, Ulrich; Zenke, Martin; Brüstle, Oliver (2012):

Industrialisierung der Herstellung von iPS-Zellen. In: Laborwelt 13 (4), S. 26–27. 

 

Lindvall, Olle; Barker, Roger A.; Brüstle, Oliver; Isacson, Ole; Svendsen, Clive N. (2012):

Clinical Translation of Stem Cells in Neurodegenerative Disorders.

In: Cell Stem Cell 10 (2), S. 151–155. DOI: 10.1016/j.stem.2012.01.009.

 

Lohmann, Michael; Walenda, Gudrun; Hemeda, Hatim; Joussen, Sylvia; Drescher, Wolf; Jockenhoevel, Stefan et al. (2012):

Donor Age of Human Platelet Lysate Affects Proliferation and Differentiation of Mesenchymal Stem Cells.

In: PloS ONE 7 (5), Artikel e37839. DOI: 10.1371/journal.pone.0037839.

 

Schellenberg, Anne; Stiehl, Thomas; Horn, Patrick; Joussen, Sylvia; Pallua, Norbert; Ho, Anthony D.; Wagner, Wolfgang (2012):

Population dynamics of mesenchymal stromal cells during culture expansion.

In: Cytotherapy 14 (4), S. 401–411. DOI: 10.3109/14653249.2011.640669.

 

Walenda, Gudrun; Hemeda, Hatim; Schneider, Rebekka K.; Merkel, Rudolf; Hoffmann, Bernd; Wagner, Wolfgang (2012):

Human Platelet Lysate Gel Provides a Novel Three Dimensional-Matrix for Enhanced Culture Expansion of Mesenchymal Stromal Cells.

In: Tissue Eng. Part C Methods 18 (12), S. 924–934. DOI: 10.1089/ten.TEC.2011.0541.